microbiology lab paper

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Hello, I was a given microbe Enterobacter Aerogenes. I performed multiple biochemical test to come to the conclusion of my specific microbe. Please see below for my microbe results and attached rubric for paper.


Paper Criteria (please see attached for more details)

  • Must use paragraph format (write as narrative)
  • MLA bibliography format
  • Length of paper 4-5 pages (less than three, not including chart, will not be accepted)
  • 1 ½ line spacing, 11 pt type font, proper grammar, punctuation, spelling, number pages
  • Refrain from the use of personal pronouns and be specific (minimize fluff)
  • Names of all genus (first word in microbe name) is capitalized and species (second name) is lower case (e.g., S. aureus)
  • Names of all taxa must be printed in italics.The first use of the name should be written out with subsequent uses abbreviating the first name (e.g., Staphylococcus aureus andS. aureus)
  • Chart size should not exceed 1/2 page
  • Identify/discuss a minimum of 5 experiments that were most helpful in the ID of your microbe
  • Explain/Interpret what the results mean or indicate
  • Discuss any variables or reasons why your results may have been contrary to book results
  • Must reference all quoted materials used
  • Cannot use class handout materials or Wikipedia as a cited reference



Results!!

-Sugar Fermentation (Lactose, Dextrose, and Sucrose)- positive for both acid and gas production.(Hence “AG” via chart attached)

-Triple suagr iron (TSI)- positive for acid production

-Methyl Red Voges Proskauer (MVRP) Test- MR Reaction was (-) no glucose breakdown /no color change, VP Reaction was (+) glucose breakdown to acetylmethylcarbinol – red ring around top of broth

-Citrate Utilization Test- (+) citrate utilization giving alkaline PH- royal blue media

-Sulfide, Indole, & Motility (SIM Test)- Sulfide- (-) no H2S to FeS/ no color change,Indole (+) indole production cherry red at top of agar, Motility was (+) due to cloudiness throughout agar

-Gelatin (Protein) Hydrolysis- (-) gelatinase was produced medium remains liquid after refrigeration.

-Starch Hydrolysis – (-) no starch hydrolysis agar is blue/blackincluding around bacterial growth

-Casein (Milk) Hydrolysis- (+) casein hydrolyzed clear zone around bacterial growth.

-Lipid Hydrolysis- (-) no lipid , opaque agar

-Urea Hydrolysis- (-) no urea/color change

-Nitrate Reduction- (+) reduced nitrates to nitric oxide, nitrous oxide or nitrogen gas, no color change

-Oxidase Test- (-) no cytochrome oxidase activity/no color change

-Catalase Test- (+) catalase production, bubbles appear

 
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